Chiral mesoporous silica nano-screws as an efficient biomimetic oral drug delivery platform through multiple topological mechanisms

In the microscale, bacteria with helical body shapes have been reported to yield advantages in many bio-processes. In the human society, there are also wisdoms in knowing how to recognize and make use of helical shapes with multi-functionality. Herein, we designed atypical chiral mesoporous silica nano-screws (CMSWs) with ideal topological structures (e.g., small section area, relative rough surface, screw-like body with three-dimension chirality) and demonstrated that CMSWs displayed enhanced bio-adhesion, mucus-penetration and cellular uptake (contributed by the macropinocytosis and caveolae-mediated endocytosis pathways) abilities compared to the chiral mesoporous silica nanospheres (CMSSs) and chiral mesoporous silica nanorods (CMSRs), achieving extended retention duration in the gastrointestinal (GI) tract and superior adsorption in the blood circulation (up to 2.61- and 5.65-times in AUC). After doxorubicin (DOX) loading into CMSs, DOX@CMSWs exhibited controlled drug release manners with pH responsiveness in vitro. Orally administered DOX@CMSWs could efficiently overcome the intestinal epithelium barrier (IEB), and resulted in satisfactory oral bioavailability of DOX (up to 348%). CMSWs were also proved to exhibit good biocompatibility and unique biodegradability. These findings displayed superior ability of CMSWs in crossing IEB through multiple topological mechanisms and would provide useful information on the rational design of nano-drug delivery systems.

Covalent organic nanospheres as a fiber coating for solid-phase microextraction of genotoxic impurities followed by analysis using GC-MS / 药物分析学报

Covalent organic nanospheres(CONs)were explored as a fiber coating for solid-phase microextraction of genotoxic impurities(GTIs)from active ingredients(AIs).CONs were synthesized by an easy solution-phase procedure at 25℃.The obtained nanospheres exhibited a high specific surface area,good ther-mostability,high acid and alkali resistance,and favorable crystallinity and porosity.Two types of GTIs,alkyl halides(1-iodooctane,1-chlorobenzene,1-bromododecane,1,2-dichlorobenzene,1-bromooctane,1-chlorohexane,and 1,8-dibromooctane)and sulfonate esters(methyl p-toluenesulfonate and ethyl p-toluenesulfonate),were chosen as target molecules for assessing the performance of the coating.The prepared coating achieved high enhancement factors(5097-9799)for the selected GTIs.The strong affinity between CONs and GTIs was tentatively attributed to T-T and hydrophobicity interactions,large surface area of the CONs,and size-matching of the materials.Combined with gas chromatography-mass spectrometry(GC-MS),the established analytical method detected the GTIs in capecitabine and imatinib mesylate samples over a wide linear range(0.2-200 ng/g)with a low detection limit(0.04-2.0 ng/g),satisfactory recovery(80.03％-109.5％),and high repeatability(6.20％-14.8％)and reproducibility(6.20％-14.1％).Therefore,the CON-coated fibers are promising alternatives for the sensitive detection of GTIs in AI samples.

Preparation and identification of folate-targeting liver cancer phase-transition nanoparticles carrying suicide gene / 肿瘤

Objective: To develop the folic acid (FA)-targeting poly (lactic-co-glycolic acid) (PLGA) phase-transition nanoparticles loaded with herpes simplex virus type(HSV1)-thymidine kinase (TK) suicide gene and perfluoropentane (PFP) (named as FA-PLGA/PFP-TK), as well as to identify the targeting performance, enhancement effect of ultrasound imaging and inhibitory effect on the proliferation of hepatocellular carcinoma cells. Methods: The FA-PLGA/PFP-TK nanoparticles were prepared by double emulsion method and carbodiimide method. The size distribution and Zeta potential of these nanoparticles were measured using a Malvern measuring instrument, and the morphological characteristic was observed by electron microscopy. The human liver cancer SMMC-7721 cells were used to verify the targeting performance of FA-PLGA/PFP-TK nanoparticles. Low intensity focused ultrasound (LIFU) was used to detect the results of ultrasound enhanced imaging and TK gene transfecting into SMMC-7721 cells. The expression of TK protein was detected by Western blotting, and the viability of SMMC-7721 cells was evaluated by CCK-8 assay. Results: The size of nanoparticles was (207.00 ± 46.06) nm with the shape of regular sphere. The entrapment efficiency of TK gene was (34.95 ± 3.14)%. In vitro targeting experiments showed the targeting nanoparticles were gathered around SMMC-7721 cells. After LIFU irradiation, the nanoparticles could enhance the ultrasound imaging, the expression level of TK protein was increased, and the viability of SMMC-7721 cells was decreased in the FA targeting group (all P < 0.01). Conclusion: FA-PLGA/PFP-TK targeting nanoparticles are prepared successfully, which is expected to be used in ultrasound imaging-guided gene targeted therapy.

Prussian blue nanosphere-embedded hydrogel for photothermal therapy by peritumoral administration

To establish an injectable hydrogel containing Prussian blue (PB) nanospheres for photothermal therapy against cancer, PB nanospheres were prepared by one-pot synthesis and the thermosensitive Pluronic F127 was used as the hydrogel matrix. The PB nanospheres and the hydrogel were characterized by shape, particle size, serum stability, photothermal performance upon repeated 808 nm laser irradiation, as well as the rheological features. The effect of the PB nanospheres and the hydrogel were evaluated qualitatively and quantitatively in 4T1 mouse breast cancer cells. The retention, photothermal efficacy, therapeutic effects and systemic toxicity of the hydrogel were assessed in a tumor-bearing mouse model. The PB nanospheres had a diameter of about 150 nm and exhibited satisfactory serum stability, photo-heat convert ability and repeated laser exposure stability. The hydrogel encapsulation did not negatively influence the above features of the photothermal agent. The nanosphere-containing hydrogel showed a phase transition at body temperature and, as a result, a long retention time . The photothermal agent-embedded hydrogel displayed promising photothermal therapeutic effects in the tumor-bearing mouse model with little-to-no systemic toxicity after peritumoral administration.

Sistemas de nanopartículas poliméricas II: estructura, métodos de elaboración, características, propiedades, biofuncionalización y tecnologías de auto-ensamblaje capa por capa (layer-by-layer self-assembly) / Polymeric nanoparticle systems: structure, elaboration methods, characteristics, properties, biofunctionalization and self-assembly layer by layer technologies

Los materiales poliméricos han sido ampliamente investigados para aplicaciones biomédicas, teniendo especial relevancia cuando se encuentran en forma de micro- y nano-partículas. Últimamente se ha ampliado su campo de aplicación al ser conjugados con péptidos y ácidos nucleicos, por lo tanto, el interés en el estudio de este tipo de materiales, así como también en la formulación de nanoestructuras funcionalizadas como materiales, dispositivos y vehículos de transporte de agentes terapéuticos ha aumentado. Las recientes investigaciones en nanosistemas se inspiran en fenómenos naturales que estimulan la integración de señales moleculares y la mimetización de procesos a nivel celular, de tejidos y órganos. Tecnológicamente, la capacidad de obtener nanoestructuras esféricas mediante la combinación de materiales que presenten propiedades distintas a las que ningún otro material individual posee por sí solo, es lo que hace que las nanocápsulas sean particularmente atractivas. Las potenciales ventajas de los sistemas de nanopartículas de tipo polimérico se destacan a lo largo de cada parte de este artículo de revisión. El presente artículo aborda los aspectos más relevantes sobre la estructura, composición y algunos métodos de elaboración de los sistemas nanoparticulados. Además, expone algunos de los trabajos más recientes, centrados en sistemas de nanopartículas basados en polímeros dirigidos a la administración de agentes, publicados en artículos especializados de investigación y revisiones durante los últimos años.

Polymeric materials have been extensively investigated for biomedical applications including micro- and nanoparticles. Modern advances have broadened horizons for application with peptides and nucleic acids. Therefore, interests increased in the formulation of materials, devices and vehicles for transporting therapeutic agents in functionalized nanostructures. Recent nano-systems are inspired by natural phenomena that stimulate the integration of molecular signals and the mimicking of natural cellular processes, at tissue and organ levels. Technologically, the ability to obtain spherical nanostructures, which combine different properties, that no other single material possesses on its own, makes nanocapsules particularly attractive. Potential advantages over polymer nanoparticulate systems are highlighted throughout each part of this review article. Here, we address the most relevant aspects of structure, composition and methods of formulation of nanoparticulate systems. In addition, we outline some of the more recent works focusing on nanosized preparations, based on agent-directed polymers, found in specialized research articles that have emerged in the recent years.

Preparation and in Vitro Anti-Laryngeal Cancer Evaluation of Protopanaxadiol-loaded Hollow Gold Nanoparticles / 分析化学

Protopanaxadiol (PPD) has inhibitory effects on many tumors and receives much attention. However,it has poor water solubility and low utilization, which limits its clinical application. Considering these issues,in this study,we used hollow gold nanoparticles as transport carriers of PPD and synthesized PPD hollow gold nanoparticles (HAuNs). We conducted a number of experiments to investigate the in vitro anti-laryngeal cancer Hep-2 effect of a PPD HAuNs carrier. High performance liquid chromatography(HPLC) was used to detect the sustained release effect of PPD HAuNs. MTT assay was used to detect the inhibitory effect of PPD HAuNs on the proliferation of Hep-2 cells. Effect of PPD HAuNs on Hep-2 cell apoptosis was investigated by flow cytometry. The results of in vitro release showed that PPD HAuNs had sustained release effect. Compared with blank control group,HAuNs group and PPD group,the survival rate of Hep-2 cells in HAuNs-PEG-PPD group decreased more significantly and the apoptosis rate increased more significantly (p<0.01). PPD HAuNs could significantly enhance anti-laryngeal cancer effect of PPD in vitro and promote the apoptosis of tumor cells. It promotes tumor cell apoptosis, and is expected to be a new PPD drug delivery system, further promoting the application of PPD in clinical anti-laryngeal cancer.

MUC1 Mucin 1 Antibody Marked Superparamagnetic Iron Oxide Nanoparticles in Nude Mice Model of Pancreatic Cancer: An Experimental Study / 中国医学影像学杂志

Purpose To prepare superparamagnetic iron oxide nanoparticles (SPION) probe targeted and modified by MUC1 murin (MUC1) in order to explore its MRI characteristics in pancreatic cancer transplantation model.Materials and Methods Chemical conjugate method was adopted for coupled response of MUC1 and SPION to construct targeted probe and tested its basic physical properties,including water and diameter,surface charge and MR signal measuring.Meanwhile,nude mice model of pancreatic cancer transplant subcutaneous sarcoma was set up to study imaging effect inside the nude mice.Transplant sarcoma specimen was taken and immunohistochemical and Western blot were adopted to measure MUC1 expression.Results Partial size of the prepared particle probe was approximately 63.5 nm and surface charge was about 10.2 mV.The probe solution could obviously decrease MR transverse relaxation time (T2 value).In vitro experiment,MUC 1 could selectively gather on nude mice transplant sarcoma model could greatly lower T2 signal intensity.Conclusion Prepared probe has small partial size,superparamagnetic and other advantages.It can realize combination with pancreatic cancer tissue specificity and provide reliable in vivo iconology in early stage for disease diagnosis through vitro imaging.

Fabrication and in vitro biological safety of a novel functional coating on microarc-oxidized titanium / 实用口腔医学杂志

Objective:To fabricate and to study the surface morphology and biological safety of a novel coating on microarc-oxidized titanium.Methods:The novel functional coating was fabricated by cross-linking the double-layer nanoparticles loading rhBMP-2 and SDF-1 with gelatin on microarc-oxidation coating on titanium implant surface.The surface topography was observed and optimized,and the biological safety of the novel coating was primarily evaluated by cell toxicity test,oral mucosa stimulation test and hemolysis test in vitro.Results:The novel functional coating possesses excellent morphology.The coating showed the cytotoxicity of score 1 and no mucous membrane irritation,the hemolytic rate of the coating was 4.6％.Conclusion:The coating possesses good morphology and biological safety.

An experimental study on the detection of Porphyromonas gingivalis with multifunctional nanospheres / 实用口腔医学杂志

Objective:To development a new method for sensitive detection of Porphyromanus gingivalis (P.gingivalis) based on magnetic encoding nanospheres and upconversion fluorescent encoding nanospheres.Methods:Magnetic and upconversion fluorescent encoding nanospheres were prepared by sol-gel method respectively,combined the monoclonal antibodies specific to P.gingivalis after modifying the surface of nanospheres.The system was used to detect P.gingivalis from mixed bacteria solution of P.gingivalis,F.nucleatum and S.mutans.Fluorescent microscopy with an external 980 nm near-infrared hght pulse laser,scanning and transmission microscope were used to evaluate the effectiveness of the detection system.Results:Magnetic and upconversion encoding nanospheres had better dispersion,particle size uniformity and homogeneous morphology.Besides,the magnetic encoding nanospheres had good magnetic properties and strong fluorescence intensity.P.gingivalis was captured by magnetic and upconversion encoding nanospheres in a mixed solution of the 3 bacteria with a detection limit of 10 CFU/ml.Conclusion:The method designed in this study can capture P.gingivalis sensitively in a mixed bacteria liquid.

Immunomagnetic Nanosphere-based Strategy for Rapid, Efficient and Accurate Detection of Lymphnode Metastatic Carcinoma Cells / 分析化学

A strategy based on immunomagnetic nanospheres ( IMNs ) for rapid, efficient and accurate detection of lymphnode metastasis carcinoma cells ( LNMCCs ) was developed in this study. First, IMNs processing magnetism and biological targeting were fabricated by the approach developed by our group previously. Then, LNMCCs in lymph node fine needle aspiration ( LNFNA) specimens were separated and enriched by the immunomagnetic isolation using IMNs. At last, the captured cells were identified with Wright's staining and immunocytochemistry ( ICC) . The separation and enrichment of LNMCCs with immunomagnetic isolation could reduce the background interference of LNFNA specimens effectively; the identification with Wright ' s staining and ICC offered more reliable information for accurate diagnosis, so the sensitivity, specificity and overall diagnostic accuracy had an obvious improvement compared with the conventional cytologic diagnosis. Besides, the simple and rapid incubation of LNFNA specimens with IMNs needed just 5 min, so the cytomorphology of captured LNMCCs could be intactly retained, which enabled to provide important basis for classifying lymphnode metastasis carcinoma ( LNMC ) and the subsequent pathological study. Moreover, the specific capture of epithelial carcinoma cells in LNFNA specimens with IMNs could make a definite diagnosis of the captured cells as LNMCCs, thus realizing the differentiated diagnosis of LNMC and malignant lymphoma. Additionally, this strategy exhibited successful LNMCCs detection in LNFNA specimens from 110 patients and had higher sensitivity ( 98 . 0%) , specificity ( 100 . 0%) , and overall diagnostic accuracy (98. 2%) than the conventional cytologic diagnosis. Therefore, it was a new attempt to use IMNs for detection of LNMCCs in LNFNA specimens from LNMC patients, and offered new ideas for LNMC diagnosis and study.

Inhibition effects of paclitaxel/NLS-KALA-SA nanoparticles on A549 cell line in vitro / 天津医药

Objective To observe NLS-KALA-SA-PTX (NKSP) for lung adenocarcinoma cell line A549 in vitro with paclitaxel monotherapy, and the mechanism thereof. Methods MTT assay was used to detect A549 cell proliferation influ-enced by different concentrations of NKSP (20, 40, 80, 100μg/L) and paclitaxel monotherapy (20, 40, 80, 100μg/L) for 24 h, 48 h and 72 h.. Subsequent experiments were divided into four groups, namely, group A (without any drug treatment), group B (added polypeptide 80μg/L of self-assembled nanoparticles, NKS), group C (80μg/L paclitaxel monotherapy) and group D (80μg/L NKSP). Flow cytometry was used to detect the cell apoptotic rates after 48 h and 72 h treatment in four groups. Western blot assay was used to analyse the protein expressions of bax and caspase-3 after 48 h and 72 h treatment in four groups. Results Both paclitaxel monotherapy and NKSP can inhibit the proliferation of A549 cells. The inhibitory rates of paclitaxel monotherapy group at 48 h and 72 h and NKSP group at 72 h showed an increasing trend in a dose-depen-dent manner (P<0.05). After treatment for 48 hours, the apoptotic rate was significantly higher in D group than that of C group (P<0.05). But the apoptotic rate at 72 h was lower in D group than that of C group (P<0.05). The protein expressions of bax and caspase-3 at 48 h were significantl lower in D group than those of C group, which were higher at 72 h in D group than those of C group (P<0.05). Conclusion Compared to paclitaxel monotherapy group, NKS promotes slow release of pa-clitaxol, which reduces the cytotoxicity and extends the antitumor effects.

In vitro and in vivo evaluation of nano-based films for buccal delivery of zolpidem

Abstract Insomnia is becoming increasingly prevalent in the world general population. Therapies used by patients include over-the-counter therapies, herbal and dietary supplements, and pharmacological or nonpharmacological treatments. Among these, zolpidem is a pharmacological treatment popularly used for insomnia. Zolpidem is well tolerated and especially efficacious for initiation of sleep, and therefore is effective for the treatment of sleep-onset insomnia. The purpose of the present study was to design and evaluate zolpidem nanoparticle-impregnated buccal films to prolong the duration of its action. Zolpidem nanospheres were prepared by double emulsion solvent evaporation and then loaded into buccoadhesive films (Z1-Z4) comprised of different concentrations of HPMC K100, Eudragit® RL 100, and carbopol 974P. The prepared films were characterized for physicomechanical properties, mucoadhesion, percent hydration, in vitro drug release, ex vivo permeation, and in vivo studies. In vitro drug release was found to depend upon film composition. Ex vivo studies showed that film Z4 had the highest flux. In vivo studies revealed that administration of zolpidem nanosphere-impregnated film enhanced absorption of the drug (p < 0.0001), with a higher peak plasma concentration (52.54 ± 8.22 ng/mL) and area under the curve from time 0 to α (236.00 ± 39.51 ng.h/mL) than oral administration. The increase in time taken to reach the maximum drug concentration (1.5 h) further signifies the potential of these films to provide prolonged drug release. Given these promising results, we concluded that these buccal films could be an alternative route for effective zolpidem delivery.

Cyclodextrin glucanotransferase immobilization onto functionalized magnetic double mesoporous core-shell silica nanospheres

Background Cyclodextrin glucanotransferase (CGTase) from Amphibacillus sp. NPST-10 was covalently immobilized onto amino-functionalized magnetic double mesoporous core-shell silica nanospheres (mag@d-SiO2@m-SiO2-NH2), and the properties of the immobilized enzyme were investigated. The synthesis process of the nanospheres included preparing core magnetic magnetite (Fe3O4) nanoparticles, coating the Fe3O4 with a dense silica layer, followed by further coating with functionalized or non-functionalized mesoporous silica shell. The structure of the synthesized nanospheres was characterized using TEM, XRD, and FT-IR analyses. CGTase was immobilized onto the functionalized and non-functionalized nanospheres by covalent attachment and physical adsorption. Results The results indicated that the enzyme immobilization by covalent attachment onto the activated mag@d-SiO2@m-SiO2-NH2, prepared using anionic surfactant, showed highest immobilization yield (98.1%), loading efficiency (96.2%), and loading capacity 58 µg protein [CGTase]/mg [nanoparticles]) which were among the highest yields reported so far for CGTase. Compared with the free enzyme, the immobilized CGTase demonstrated a shift in the optimal temperature from 50°C to 50-55°C, and showed a significant enhancement in the enzyme thermal stability. The optimum pH values for the activity of the free and immobilized CGTase were pH 8 and pH 8.5, respectively, and there was a significant improvement in pH stability of the immobilized enzyme. Moreover, the immobilized CGTase exhibited good operational stability, retaining 56% of the initial activity after reutilizations of ten successive cycles. Conclusion The enhancement of CGTase properties upon immobilization suggested that the applied nano-structured carriers and immobilization protocol are promising approach for industrial bioprocess for production of cyclodextrins using immobilized CGTase.

Effects of targeted treatment of the carboplatin-Fe@C nanocage-loaded chitosan nanoparticles on rats with transplanted liver cancer / 肿瘤研究与临床

Objective To investigate the effects of targeted treatment of the carboplatin-Fe@C nanocage-loaded chitosan nanoparticles (C-Fe@CN-CN) combining external magnetic field on rats with transplanted liver cancer.Methods Twenty-four model rats with transplanted liver cancer were established and divided into four groups randomly (n =6).Abdominal exposure was carried out through a midline incision,and a cannula was inserted into the hepatic artery and fixed.Group A:saline water was injected as control,group B:saline water with 10 mg/kg free carboplatin was given,group C:saline water with C-Fe@CN-CN (equivalent dose of free carboplatin 10 mg/kg) was injected in absence of magnetic field,group D:saline water with C-Fe@CN-CN (equivalent dose of free carboplatin 10 mg/kg) was injected in presence of magnetic field for 30 min.All the animals were sacrificed and abdominal exposure was done again after 7 days.After tumors were reselcted,tumor weight and volume was measured,the inhibiting rate of tumor weight was calculated.Tumor and liver tissues were examined for histological changes.Results The growth of tumor was significantly inhibited after therapy with different forms of carboplatin.There was significant difference in the tumor weight of A,B,C,D groups [(0.85±0.12) g,(0.61±0.10) g,(0.48±0.09) g,(0.33±0.06) g,P ＜ 0.05,respectively].The inhibiting rates of tumor weight of B,C,D groups were 28.9 ％,43.4 ％,61.7 ％ respectively.The inhibiting rate of D group was highest which was 1.1 times higher than that of B group.There was also significant difference in the tumor volume of A,B,C,D groups [(1.06±0.24) cm3,(0.72±0.10) cm3,(0.50±0.07) cm3,(0.28±0.05) cm3,P ＜ 0.05,respectively].The tumor volume of group A was largest which was 2.8 times larger than that of group D.In group D,tumor tissues from six rats presented severe necrosis,and nanoparticles were concentrated in the necrotic tissue.In group C,five rats presented middle necrosis,one rats presented severe necrosis.There was no concentration of nanoparticles in the necrotic tissue.In group B,four rats presented middle necrosis,two rats presented mild necrosis.In group A,six rats presented mild necrosis.Conclusion C-Fe@CN-C can significantly increase the therapeutic effects of carboplatin by hepatic artery injection combining with an external magnetic field on the tumor.

Effect of FK506 nanospheres on regeneration of allogeneic nerve after transplant

OBJECTIVE@#To discuss effect of FK506 nanospheres used at different time on the regeneration of allogeneic nerve after transplant.@*METHODS@#Single emulsion-solvent evaporation method (O/W) was adopted to prepare the FK506 nanospheres and the tibial nerve of rats after allogeneic transplantation. FK506 nanospheres were used in group A after operation immediately, in group B in 24 h after operation, and in group C in 3 d after operation while FK506 nanospheres were not used in group D; in the 4th, 8th and 12th week after operation respectively, general observation of transplanted nerves, histological examination, image analysis of myelinated fibers, wet-weight determination of musculi triceps surae, retrogradely labeling of neurons by the fluorescein and electrophysiological comparison of bilateral tibial nerve were carried out.@*RESULTS@#FK506 nanospheres can be degraded and absorbed quickly. The neural regenerations in group A and B were similar, which were both much better than those in group C and D. The difference was statistically significant and so was the difference between group C and D.@*CONCLUSIONS@#Drug release rate of FK506 nanospheres is accordant with the regeneration law of damaged nerves and the local application can promote the regenerations of nerves. The effect would be better if the drug is used in earlier period (within 24 h).

Influence of hydroxyapatite nanoparticle-Mg2+ on the gene transfection to colorectal cancer cell line SW480/M5 / 肿瘤研究与临床

Objective To investigate the role of hydroxyapatite nanoparticle (nHAP) in the gene transfection of human colorectal cancer cell line SW480/M5 and the possible mechanisms.Methods The combination and protection of nHAP-Mg2+ to DNA were analyzed by gelose gelatin electrophoresis.Liposome and nHAP modified by magnesium chloride was combined,and the PEGFP-N1 plasmids were transfected into SW480/M5 cells.The gene transfection rate and the mean fluorescence intensity were observed by flow cytometry.The effect of nHAP-Mg2+ on the growth of the cells were studied by MTT.Results At appropriate proportion,nHAP-Mg2+ could combine the plasmids compeletly and protected the DNA.The gene could not be transferred by nHAP-Mg2+ alone.Combining the nanoparticles and liposome,the gene could be transferred very efficiently and the transfection rates were significantly higher than the liposome (P ＜ 0.05).The inhibition of cell growth was increased along with the concentration of nHAP-Mg2+ wether it was used alone or with the combination of liposome (P ＜ 0.05).Conclusions nHAP-Mg2+ has the ability to combining and protecting DNA and can be used to transfer gene as the adjunct carrier of liposome for the gene therapy of tumor cells to elevate the gene tansfection and expression rate and also enhance the anti-tumor effection.

Effect of basic fibroblast growth factor-polylactide sustained release nanospheres on proliferation and adipogenic induction of adipose-derived stem cells in vitro / 中华医学美学美容杂志

ObjectiveTo investigate the effect of basic fibroblast growth factor-polyactide release nanospheres on proliferation and adipogenic induction of adipose-derived stem cells in vitro.MethodsAdipose-derived stem cells were isolated and induced for three-line differentiation in vitro.The culture medium and inductive medium of stem cells were prepared containing 0,1,2,3,4 and 5 mg/ml basic fibroblast growth factor-polyactide release nanospheres,respectively.Adipose-derived stem cells were cultured ina 96-well plate and replaced the culture medium containing release nanospheres the second day.The cells proliferation was detected by the method of MTT every day and quanti fication of oil red O every other day.The data obtained were analyzed with SPSS13.0 statistical software.ResultsThe basic fibroblast growth factor polyactide release nanospheres had the ability promoting proliferation and adipogenic induction of adipose-derived stem cells.The best concentration of nanospheres was 3 mg/ml and 4 mg/ml,respectively.ConclusionsThe basic fibroblast growth factor-polyactide release nanospheres could promote proliferation and adipogenic induetionof adipose-derived stem cells significantly.It could be used as an ideal cytokine release nanospheres in adipose tissue engineering.

Co-polymeric hydrophilic nanospheres for drug delivery: Release kinetics, and cellular uptake.

Nanobiotechnology focuses on the biological effects and applications of nanoparticles that include nano-safety, drug encapsulation and nanotherapeutics. The present study focuses on hydrophilic nanospheres of copolymers N-isopropylacrylamide [NIPAAM] and vinyl pyrrolidone [VP], encapsulating a bioactive derivative of 5-fluorouracil-hexyl-carbamoyl fluorouracil (HCFU). The size of the nanospheres was ~58 nm and the surface charge measured was -15.4 mV. Under optimal conditions, the yield was >80%, and the drug loading was 2%. The entrapment efficiency was ~75%. Wide-angle X-ray diffraction analysis showed that the entrapped HCFU was present in an amorphous state, which has higher water solubility compared with the crystalline state. Slow drug release from nanospheres was observed in PBS and serum, with ~80% released at 37°C after 72 h. The HCFU loaded polymeric nanospheres have been found to be stable in whole blood having negligible RBC toxicity. Cytotoxicity in Mia-Paca 3, pancreatic cancer cell line was done in a 24-72 h assay. Dose dependant cytotoxicity was observed when incubated with various concentrations of HCFU loaded polymeric nanospheres while HCFU per se (<1 mg) showed 90% toxicity within 24 h.

The effect of 5-FU polylactic acid nanospheres on the proliferation and apoptosis of human tongue squamous cell carcinoma Tca83 cells / 实用口腔医学杂志

Objective:To study the effect of 5-FU polylactic acid nanospheres(5-FU-PAN)on the proliferation and apoptosis of the human tongue squamous cell carcinoma Tca83 cells.Methods:Tca83 cells were exposed to 5-FU-PAN or 5-FU at the doses(mmol/L)of 1?10-2,2?10-2 and 4?10-2 for 1,3,5,7,9 and 11 days respectively.The survival rates of the cells were examined by MTT assay,the apoptosis of the cells was observed by acridine orange fluorescent staining and the cell cycle distribution was studied by flow cytometry.Results:Both 5-FU-PAN and 5-FU inhibited the proliferation and promoted the apoptosis of the cells dose and time dependantly.5-FU-PAN showed stronger effects than 5-FU.The two agents blocked the cells at S phase and 5-FU-PAN was more potential than 5-FU.Conclusions:5-FU-PAN is more effective in the growth inhibition and apoptosis promotion of Tca83 cells.

Mechanism of breviscapine entrapped by polymethacrylate nanospheres / 中成药

AIM: To prepare polymethacrylate nanospheres and study the mechanism of breviscapine entrapped by polymethacrylate nanospheres. METHODS: Polymethacrylate nanospheres were prepared by microemulsion polymerization with SDS as surfactant, n-butanol as cosurfactant, MAA and BMA as monomer, TRIM as cross-linker and AIBN as initiator. Breviscapine added would be divided into two ways:prior to polymerization (encapsulation) and after polymerization (sorption), respectively. RESULTS: The size of the nanospheres was found to be 50nm by measuring with TEM. The ? potential of the nanospheres was -27.2 mv and it was increased after breviscapine being entrapped. The drug content entrapped in nanospheres was proportional to the drugs amount added in encapsulation method while the drug content in nanospheres was increased step by step in sorption method. CONCLUSION: The polymethacrylate nanospheres prepared by microemulsion polymerization could be applied to encapsulate hydrophobic traditional Chinese medicine extracts.